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KMID : 0903520000430030169
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2000 Volume.43 No. 3 p.169 ~ p.173
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Isolation , Identification and Enzyme Properties of a Bacterium producing Alkaline Protease
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Abstract
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For the development of enzyme detergent capable of effectively washing at low temperature, a bacterium producing alkaline protease was isolated from soil samples, and properties of the enzyme were investigated. The selected strain was Gram negative, rod shape(0.6∼0.7×1.3∼2.6 ㎛ in size) and motile. It had the degradation activity of aesculin, gelatin and casein, and was catalase-positive. The cell wall components was meso-DAP, and G+C mole contents was 43.3%. From these results, the strain was identified as Acinetobacter sp. KN-27. The activity of alkaline protease by this strain peaked with 3,300 D.U/mL after 36 hours in the liquid culture at 40℃. The optimal pH and temperature of the enzyme were pH 9 and 60℃, respectively. Alkaline protease produced by Acinetobacter sp. KN-27 has shown two active bands on the electrophoresis of native gel.
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